Eppendorf S41i Incubator Shaker - Manual (Page 62)

S41i CO2 Incubator Shaker — Operating manual
62
6 Maintenance
6 Maintenance
6.1 Routine maintenance
6.1.1 General notes
To ensure that chamber conditions remain as stable as possible, be sure to minimize the length
of time that the door is open. When you open the door, wipe off any small drops of condensate
that may have formed on the inner seal. This will avoid a build-up of condensation.
If you are using the humidity tray for humidification, be sure to follow the indications provided
(see Electrical connections on p. 17) and (see Using the humidity trays on p. 22).
6.1.2 Daily checks
1. Check that the temperature and CO
2
levels are reading within specification.
2. Check that gas supply lines have inline regulators, that they are properly installed and
functioning.
3. Check the reserve pressure in the CO
2
cylinder (normally 725 psi or 50 bar when full). The
design of the incubator/shaker ensures very low consumption of CO
2
: during normal working
conditions, a typical large cylinder should last approximately 12 months (frequent door
openings will deplete the supply more rapidly). If there is a significant drop at the cylinder
pressure of 725 psi or 50 bar, it means that the cylinder is almost empty and should be
replaced. Ensuring that there are no leaks at any of the connections will ensure a greater
lifetime to the CO
2
supply and will help avoid accidentally running out of CO
2
.
4. Check the ALARM and EVENT LOG screens for any alarms or events that may have
occurred overnight.
6.1.3 Weekly checks
 If you are using them for humidification, check the humidity trays and refill them as needed.
Be careful not to exceed the maximum volume of 250 mL per pan. The use of warm water
(~ 37.0 °C) will ensure a rapid return to optimum chamber conditions.
6.1.4 Monthly check
 Auto-Zero the CO
2
system at least once monthly to ensure that the CO
2
level is correct.
 We recommend monthly replacement of the water in the humidity trays. Clean the trays at the
same time.
 If required, you can take a sample of the gas inside the chamber using the CO
2
sample port,
and check it using a CO
2
gas analyzer (see CO
2
Sampling with Analyzer on p. 62).
6.1.5 Other checks
 At least once every six months, check tubing for wear and for leaks. Replace as needed.
6.1.6 CO
2
Sampling with Analyzer
We recommend that you perform a CO
2
Auto-Zero prior to sampling.
The CO
2
sample port is located on the righthand side of the incubator/shaker, near the top.
If you conduct a sampling, please ensure the following:
 Turn off the CO
2
gas by re-programming the setpoint for CO
2
to 0.0 % to prevent CO
2
from
being injected into the chamber and giving a false reading.
 A flow rate  0.5 liters/minute is used to take a sample.
 The door is kept closed to minimize heat loss.
Hint!
There is no need to remove the top panel for normal maintenance or servicing, so if you have two
incubator/shakers stacked, the upper incubator/shaker does not have to be moved when you are
servicing the lower incubator/shaker.
6
Maintenance