Eppendorf S41i Incubator Shaker - Manual (Page 22)

S41i CO2 Incubator Shaker — Operating manual
22
5 Operation
5 Operation
5.1 Preparing for operation
1. Remove the black protective cover from the CO
2
sensor, and store it in a safe place. The
sensor cap should be placed back on the sensor when the incubator/shaker is to be cleaned.
2. Ensure that the white porous sensor cover remains in place.
3. Using the mains/power cord provided, connect the incubator/shaker to a earthed/grounded
mains/power supply.
4. Switch the incubator/shaker ON using the on/off switch at the top left side of the incubator/
shaker.
The display will illuminate immediately.
5. Turn on the CO
2
gas supply with the pressure regulator set to 7 PSI (0.5 bar).
6. The chamber setpoints are pre-programmed at 37.0 °C and 5 % CO
2
. Leave the incubator/
shaker on until the programmed chamber temperature and CO
2
concentration have been
reached.
7. Leave the incubator/shaker running overnight to allow conditions to stabilize.
5.2 Using the humidity trays
If humidification is required, each of the two humidity trays should be filled with approximately
250 mL of warm (~ 37.0 °C) distilled water at this time.
The humidity level within the chamber is not adjustable. The internal chamber will reach between
85 and 95 % relative humidity at 37 °C (depending on ambient humidity) using the humidity
trays.
5.3 Starting the incubator/shaker
To initially start the incubator/shaker, close the door, make sure the incubator/shaker’s mains/
power cord is securely plugged in, and turn the mains/power switch (located on the top left side of
the instrument) to the ON (I) position.
CAUTION!
Risk of silica particulates transferring to skin
 Wear gloves anytime you touch the white CO
2
sensor cover.
 Do not later touch those gloves to your face.
 Discard or wash the gloves.
Hint!
• The incubator shaker’s CO
2
valve is disabled until the incubator/shaker reaches the
temperature setpoint. After the temperature setpoint is reached, the CO
2
valve is activated,
allowing the incubator/shaker to reach the CO
2
setpoint.
• If mains/power is interrupted to the incubator/shaker long enough for the temperature to drop
below setpoint, the CO
2
valve will be deactivated until temperature setpoint is again achieved.
(This serves to avoid spurious CO
2
readings while the incubator/shaker is below its
temperature setpoint)
NOTICE!
Risk of material damage
 To avoid possible damage to the CO
2
sensor, never leave water in the humidity trays while the
incubator/shaker is switched off, or when a high temperature disinfection cycle is initiated.
 Allow a clearance of 50 mm (2 in) to allow access for oxygen sensor removal (optional
feature).
Hint!
The incubator/shaker will not operate if the door is open.
5
Operation