Luminex FLEXMAP 3D User Manual - Manual (Page 34)

Electronics
USB 2.0-compatible communications link for fast data transfer
Input voltage range: 100-120 V~, 6.0 A, 50/60 Hz or, 200-240 V~, 3.0 A, 50-60 Hz
Optics
Reporter channel detection: A/D resolution 16 bits
Reporter channel dynamic
range: ≥ 4.5 decades of detection (verified with beads dyed with high levels
of organic dyes)
Reporter laser: 532 nm, nominal output 15.0+5%/-0% mW, diode-pumped; mode of
operation, continuous wave (CW); maximum output power 50 mW
Classification laser: 638 nm, nominal output 12.0 to 12.5 mW, diode; mode of operation,
continuous wave (CW); maximum output power 15 mW
Reporter detector: Photomultiplier tube, detection bandwidth of 565 to 585 nm
Classification detector: Avalanche photo diodes with temperature compensation
Doublet discrimination
detector: Avalanche photo diodes with temperature compensation
Fluidics
Cuvette: 200 micron square flow channel
Sample injection rate: 2μL/second
Sample uptake volume: 20 to 200μL
Sheath flow rate: 7.9 (+/- 0.9) mL/min
Sheath pressure: 8 to 13 psi for normal operations; 15 psi maximum
Microspheres
Distinguishes 1 to 500 unique xMAP microspheres in a single sample
Classification of xMAP
microspheres: ≥ 80%
Total system
misclassification of xMap
microspheres: ≤ 4%
Internal sample carryover: < 1.5%
Detects a minimum of 500 fluorochromes of phycoerythrin (PE) per xMAP microsphere
Luminex ® FLEXMAP 3D ® Hardware User Manual
24
For research use only. Not for use
in diagnostic procedures.