Bio-Rad Ready Gel Precast Gels for 2-D Electrophoresis - Manual (Page 8)

Section 2
Setup and Basic Operation Using Mini-PROTEAN Tetra Cell
2.1 Setting Up and Running Ready Gel Precast Gels
1. Each Ready Gel should be used immediately after it is removed from the storage pouch.
2. Remove the comb and gently rinse the wells with deionized water or running buffer.
3. Use the key knife or a razor blade to cut the tape at the bottom of the gel along the black “cut here” line.
It is helpful to cut all the way to the edge of the cassette where the pull tab begins.
4. Pull the tape tab along the cut line, up from the cassette and at an angle towards the comb end of the
gel.
Required materials:
• Clean and dry Mini-PROTEAN® Tetra cell tank
• Electrophoresis module (Electrode Assembly Module only for 1 or 2 gels; for 3 or 4 gels also use the
Companion Running Module)
• Running buffer (700 ml for 2 gels; 1000 ml for 4 gels)
• Ready Gel® precast gels or hand-cast gels
• PowerPac™ Basic power supply
1. Assembly
3
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