Bio-Rad Ready Gel Precast Gels for 2-D Electrophoresis - Manual (Page 18)

Section 5
Peptide Analysis
5.1 Introduction
Ready Gel Tris-Tricine/peptide gels are optimized for separating peptides and proteins <10 kD. Superior
resolution of peptides is achieved by moving the peptide-SDS complexes more slowly through the gel. This
allows the faster moving SDS micelles, which normally interfere with peptide separations, to completely
separate from the peptides, allowing distinct peptide bands to resolve.
5.2 Ready Gel Tris-Tricine/Peptide Gel Composition
Gel buffer 1.0 M Tris-HCl, pH 8.45
Cross-linker 2.6% C
Stacking gel 4% T, 2.6% C
Storage buffer 1.0 M Tris-HCl, pH 8.45, NaN
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Shelf life 12 weeks from the date of manufacture
5.3 Ready Gel Tris-Tricine/Peptide Gel Selection Guide
Tris-Tricine/peptide gels are available in either a single percentage gel or a linear gradient gel.
Peptide Gel Optimal Separation
16.5% 15–30 kD
10–20% 1–40 kD
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