Bio-Rad Ready Gel Precast Gels for 2-D Electrophoresis - Manual (Page 16)

4.3 Ready Gel Tris-HCl Gel Selection
Native PAGE separates by charge to mass ratio, making individual protein migration protein dependent.
Optimal Tris-HCl gel percentages will have to be determined experimentally.
4.4 Native PAGE Buffers
Running Buffer Working Concentration 10x Stock
25 mM Tris Tris base 15.0 g
192 mM glycine Glycine 72.0 g
to 500 ml with deionized water 87.0 g
Note: running buffer should be
~ pH 8.3. Do not adjust the pH.
Sample Buffer 2X Working Concentration 2X Stock
62.5 mM Tris-HCl, pH 6.8 0.5 M Tris-HCl, pH 6.8 1.0 ml
25% glycerol Glycerol 2.0 ml
1% Bromophenol Blue 1% Bromophenol Blue 1.0 ml
Deionized water 4.92 ml
8.0 ml
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