Bio-Rad Ready Gel Precast Gels for 2-D Electrophoresis User Manual
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Section 5 Peptide Analysis.................................................................................................. 11
5.1 Introduction......................................................................................................................................... 11
5.2 Ready Gel Tris-Tricine/Peptide Gel Composition.................................................................................. 11
5.3 Ready Gel Tris-Tricine/Peptide Gel Selection Guide............................................................................. 11
5.4 Tris-Tricine/Peptide Buffers.................................................................................................................. 12
5.5 Sample Preparation............................................................................................................................. 12
5.6 Running Conditions............................................................................................................................ 12
Section 6 Isoelectric Focusing............................................................................................ 13
6.1 Introduction......................................................................................................................................... 13
6.2 Ready Gel IEF Gel Composition ......................................................................................................... 13
6.3 Ready Gel IEF Gel Selection Guide......................................................................................................13
6.4 IEF Buffers...........................................................................................................................................14
6.5 Sample Preparation............................................................................................................................. 14
6.6 Running Conditions............................................................................................................................. 14
Section 7 Protease Analysis by Zymogram PAGE................................................................15
7.1 Introduction..........................................................................................................................................15
7.2 Ready Gel Zymogram Gel Composition...............................................................................................15
7.3 Ready Gel Zymogram Gel Selection Guide..........................................................................................15
7.4 Zymogram Gel Buffers.........................................................................................................................16
7.5 Sample Preparation............................................................................................................................. 16
7.6 Running Conditions............................................................................................................................. 16
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