Bio-Rad Ready Gel Precast Gels for 2-D Electrophoresis - Manual (Page 33)

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30–60 min.
10.5 TBE
Gel Staining
Use the following table as a guide to select an appropriate staining method.
Method Sensitivity Optimal Protein Load Advantages Disadvantages
Ethidium bromide 50 ng ~0.10 µg/band Classic fluorescent DNA stain Carcinogenic
Silver stain 1.0–2.0 ng ~0.5 µg/band More sensitive than Multiple steps
ethidium bromide
10.6 TBE-Urea Gel Staining
Samples on denaturing nucleic acid gels can be detected using multiple methods. Use the following table as
a guide to select an appropriate staining method.
Method Sensitivity Optimal Protein Load Advantages Disadvantages
Ethidium bromide 50 ng ~0.10 µg/band Classic fluorescent DNA stain Carcinogenic
Radiant
®
Red 10 ng ~0.10 µg/band Fast single-step protocol RNA and ssDNA
only
Silver stain 1.0–2.0 ng ~0.5 µg/band More sensitive than Multiple steps
ethidium bromide
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