Bio-Rad Ready Gel Precast Gels for 2-D Electrophoresis User Manual
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Table of Contents
Section 1 General Information.............................................................................................. 1
1.1 Introduction.......................................................................................................................................... 1
1.2 Ready Gel System Specifications......................................................................................................... 2
1.3 Ready Gel Comb Configurations.......................................................................................................... 2
Section 2 Setup and Basic Operation................................................................................... 3
2.1 Setting Up and Running Ready Gel Precast Gels................................................................................. 3
Section 3 SDS-PAGE............................................................................................................. 7
3.1 Introduction.......................................................................................................................................... 7
3.2 Ready Gel Tris-HCl Gel Composition.................................................................................................... 8
3.3 Ready Gel Tris-HCl Gel Selection Guide............................................................................................... 8
3.4 SDS-PAGE Buffers............................................................................................................................... 9
3.5 Sample Preparation.............................................................................................................................. 9
3.6 Running Conditions.............................................................................................................................. 9
Section 4 Native PAGE..........................................................................................................10
4.1 Introduction..........................................................................................................................................10
4.2 Ready Gel Tris-HCl Gel Composition....................................................................................................10
4.3 Ready Gel Tris-HCl Gel Selection Guide ..............................................................................................11
4.4 Native PAGE Buffers.............................................................................................................................11
4.5 Sample Preparation............................................................................................................................. 12
4.6 Running Conditions ............................................................................................................................ 12
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