Bio-Rad Ready Gel Precast Gels for 2-D Electrophoresis - Manual (Page 24)

Section 8
Nondenaturing Nucleic Acid PAGE
8.1 Introduction
Ready Gel TBE gels are ideal for separating small dsDNA fragments, especially PCR products. The uniform
nature of DNA molecules provides samples with near-uniform charge to mass ratio, allowing nondenaturing
nucleic acid PAGE to separate dsDNA by mass using a continuous TBE buffer system.
8.2 Ready Gel TBE Gel Composition
Gel buffer 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH 8.3
Cross-linker 3.3% C
Stacking gel 4% T, 3.3% C
Storage buffer 89 mM Tris, 89 mM boric acid, 2 mM EDTA, NaN
3
Shelf life 12 weeks from the date of manufacture
8.3 Ready Gel TBE Gel Selection Guide
Ready Gel TBE gels are available in a selection of single percentages and gradients for the separation of
dsDNA.
TBE Gels Optimal Separation TBE Gradient Gels Optimal Separation
5% 200–2,000 bp 4–20% 10–2,000 bp
10% 50–1,500 bp
15% 20–1,000 bp
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