Bio-Rad Ready Gel Precast Gels for 2-D Electrophoresis - Manual (Page 20)

Section 6
Isoelectric Focusing
6.1 Introduction
Ready Gel IEF gels are cast with Bio-Rad’s Bio-Lyte ® ampholytes, amphoteric molecules that set up a pH
gradient across the gels. Proteins migrate in IEF gels to their neutral isoelectric point (pI), where the protein
has zero net charge. Ready Gel IEF gels contain no denaturing agents, so all focusing is performed under
native conditions.
6.2 Ready Gel IEF Gel Composition
Gel buffer 2% ampholyte, pH 3–10, 5–8
Cross-linker 3.0% C
Stacking gel None
Storage buffer Deionized water, NaN
3
Shelf life 26 weeks from the date of manufacture
6.3 Ready Gel IEF Gel Selection Guide
Ready Gel IEF gels are available in narrow and broad pH ranges.
IEF gel pH Range
5–8 5–8
3–10 4–8.5
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