Bio-Rad Ready Gel Precast Gels for 2-D Electrophoresis - Manual (Page 26)

Section 9
Denaturing Nucleic Acid PAGE
9.1 Introduction
Ready Gel TBE-urea gels are ideal for separating small ssDNA and RNA fragments. Applications include
oligonucleotide analysis, RNase protection assays, and northern blotting.
9.2 Ready Gel TBE-Urea Gel Composition
Gel buffer 89 mM Tris, 89 mM boric acid, 2 mM EDTA, 7 M urea, pH 8.3
Cross-linker 3.3% C
Stacking gel 4% T, 3.3% C
Storage buffer 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH 8.3, NaN
3
Shelf life 8 weeks from the date of manufacture
9.3 Ready Gel TBE-Urea Gel Selection Guide
Ready Gel TBE-urea gels are available in a range of single percentage gels.
TBE-Urea Optimal Separation
5% 50–1,000 bases
10% 25–300 bases
15% 10–50 bases
21
LIT188H:LIT188G.qxd 3/12/2009 9:13 AM Page 21