Bio-Rad Ready Gel Precast Gels for 2-D Electrophoresis - Manual (Page 37)

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11.4 Zymogram Staining Solutions
Renaturation Buffer
Final Concentration
Triton X-100 25 g 2.5 %
Deionized water 900 ml
to 1 L with deionized water
Development Solution
Final Concentration
Tris base 6.06 g 50 mM
NaCl 11.7 g 200 mM
CaCl
2
(anhydrous) 0.56 g 5 mM
30% Brij-35 0.67 ml 0.02%
Dissolve in 900 ml deionized water, adjust to 7.5 with 6 N HCl, make to 1 L with deionized water.
Staining Solution
Final Concentration
Methanol 400 ml 40%
Acetic acid 100 ml 10%
Coomassie Blue R-250 5 g 0.5%
Deionized water 500 ml
Dissolve Commassie R-250 in Methnaol/acetic acid. Add deionized water to final volume of 500 ml.
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