Bio-Rad Ready Gel Precast Gels for 2-D Electrophoresis - Manual (Page 34)

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Section 11
Stock and Staining Solutions
11.1 Stock Solutions
0.5 M Tris-HCl, pH 6.8
6.06 g Tris base
~60 ml deionized water
Adjust to pH 6.8 with HCl. Make to 100 ml with deionized water and store at 4°C.
10% SDS
Dissolve 1 g SDS in water with gentle stirring and bring to 10 ml with deionized water.
1% Bromophenol Blue
Dissolve 0.1 g of Bromophenol Blue in 10 ml deionized water with gentle stirring.
1% Xylene Cyanole FF
Dissolve 0.1 g of Xylene Cyanole FF in 10 ml deionized water with gentle stirring.
0.5 M EDTA
18.6 g of EDTA
~ 50 ml of deionized water
Adjust to pH 8.0 with 1 N NaOH. Make to 100 ml with deionized water and store at 4°C.
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