Bio-Rad Ready Gel Precast Gels for 2-D Electrophoresis - Manual (Page 10)

The arms of the clamping frame push the short plates of each gel cassette up against the notch in
the green gasket, creating a leak-proof seal (check again to make certain that the short plates sit just
below the notch at the top of the green gasket). At this point, the sample wells can be washed-out
with running buffer, and sample can be loaded (Figure 4d).
Note: If running more than 2 gels, repeat steps 1a–d with the Companion Running Module.
Important Note: Do not attempt to lock the green arms of the clamping frame, without first ensuring
that the gel cassettes are perfectly aligned and stabilized against the notches on the green gaskets of the
module. To prevent the gels from shifting during the locking step, firmly and evenly grip them in place
against the core of the module with one hand.
CAUTION: When running 1 or 2 gels only, DO NOT place the Companion Running Module
in the tank. Doing so will cause excessive heat generation and prevent
electrophoretic separation.
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4a 4b
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