Bio-Rad Ready Gel Precast Gels for 2-D Electrophoresis - Manual (Page 25)

8.4 Nondenaturing Nucleic Acid PAGE Buffers
Running Buffer Working Concentration 10x Stock
50 mM Tris Tris base 0.06 g
89 mM boric acid Boric acid 27.5 g
5 mM EDTA 0.5 M EDTA (pH 8.0) 0.1 ml
to 500 ml with deionized water
Note: TBE running buffer should be
~ pH 8.3. Do not adjust the pH.
Sample Buffer 2X Working Concentration
50 nM EDTA
Tris Base 0.06 g
25% glycerol 0.5 M EDTA 0.1 ml
0.2% Bromophenol Blue Glycerol 2.5 ml
0.2% Xylene Cyanole FF 1% Bromophenol Blue 2.0 ml
1% Xylene Cyanole FF 2.0 ml
Make up to 10 ml with deionized water
8.5 Sample Preparation
Determine the desired DNA concentration of your sample based on the detection method used. (See
section 10.5 for approximate stain sensitivities.) Dilute 1 part sample with 4 parts sample buffer (see section
8.4).
8.6 Running Conditions
Power conditions 100 V constant
Starting current:
13 mA/gel
Final current: 11 mA/gel
Run time 45–105 min
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