Bio-Rad Ready Gel Precast Gels for 2-D Electrophoresis - Manual (Page 13)

3.2 Ready Gel Tris-HCl Gel Composition
Gel buffer 0.375 M Tris-HCl, pH 8.8
Cross-linker 2.6% C
Stacking gel 4% T, 2.6% C
Storage buffer 0.375 M Tris-HCl, pH 8.8
Shelf life 12 weeks
3.3 Ready Gel Tris-HCl Gel Selection Guide
Tris-HCl gels are available in a wide selection of single percentages and gradients for the separation of
proteins by SDS-PAGE.
Tris-HCl Gels Optimal Separation Tris-HCl Gradient Gels Optimal Separation
5% 100–250 kD 4–15% 20–250 kD
7.5% 40–200 kD 4–20% 10–200 kD
10% 30–150 kD 8–16% 20–120 kD
12% 20–120 kD 10–20% 10–100 kD
15% 10–100 kD
18% 10–50 kD
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