Bio-Rad Ready Gel Precast Gels for 2-D Electrophoresis - Manual (Page 6)

Section 1
General Information
1.1 Introduction
Ready Gel
®
precast gels greatly simplify polyacrylamide gel electrophoresis. They are specifically for use with
the Mini-PROTEAN Systems (Mini-PROTEAN Tetra, Mini-PROTEAN-III and Mini-PROTEAN Dodeca Cells).
Stringent production and quality control criteria, and the use of the highest quality reagents, ensure repro-
ducible electrophoretic analysis with minimum effort. Every gel is checked during production for defects, and
each lot of gels is further tested by electrophoresis to verify quality.
Ready Gel precast gels come ready to use with preformed sample wells and a stacking gel when necessary.
Each Ready Gel Cassette is 8 x 10 cm (H x W) and 4.0 mm thick. Gel dimension is 6.8 x 8.6 cm (HxW) and
1.0 mm thick. Each gel is individually packaged in a leakproof pouch with an absorbent pad containing gel
buffer and 0.02% Sodium Azide.
Ready Gel precast gels are available for use in Tris-glycine (Tris-HCl and zymogram gels), Tris-Tricine, TBE,
TBE-urea, and IEF buffer systems. The Tris-HCl gels can be used for SDS-PAGE and non-SDS gel electro-
phoresis. The Tris-Tricine/peptide gels are optimized for peptide electrophoresis. The TBE gels are for use in
nucleic acid electrophoresis and can be used for native protein electrophoresis. TBE-urea gels provide
denaturing conditions for nucleic acids. Resolution of different size ranges of proteins or nucleic acids can
be obtained by choosing the correct gel.
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