Eppendorf PatchMan NP 2 - Manual (Page 25)

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Section 5.1 provides a brief summary of how the commands can be used for
micromanipulation. Section 5.2 provides additional information on the device's mode of
operation. Sections 5.3 and 5.4 set out detailed descriptions of all the commands.
Precondition
Prior to performing the first micromanipulation, it is assumed that there is a capillary in the
correctly positioned capillary holder (see section 4.3.2) or a correctly positioned preamplifier
with capillary (see section 4.3.4).
Other requirements: The device is properly initialised (mounting side defined, etc.).
The PatchMan NP 2 micromanipulator is switched on and is in the following "start mode": The
capillary is at the top position and to the side of the sample. The LED of the "Speed" key is lit in
FINE mode.
On the microscope table is a focussed sample, e.g. cells in a petri dish, coated with medium.
Movement towards sample
Toggle "Speed" key to COARSE mode.
The capillary is moved to the surface of the medium by pushing/rotating the
joystick in the direction of the sample. Visual monitoring of the movement.
The speed can be increased by turning the wheel on the right
hand side of the control board.
The change of speed is indicated acoustically.
If the capillary touches the medium surface for example (visually monitored),
switch to FINE mode.
The capillary is moved into the beam path of the microscope by changing the
plane of the joystick. Carefully lowering the capillary by rotating the joystick to the
right brings the tip of the capillary into the focal plane (microscopic control). Adjust
the speed as necessary by way of the turn wheel.
For extra fine movements with extreme enlargement, the speed can be reduced
further by means of the Extra Fine mode. In this mode, the turn wheel can be used
to set five different speeds.
5.1 First steps
Coarse
Fine
Coarse
Fine
Extra
Fine
User control and mode of operation
5 User control and mode of operation
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