Multichannel Systems MEA Manual - Manual (Page 47)

Recording with MEAs
47
6 Culture Chamber and Ring Options
You have several options regarding culture chamber interface rings (without ring, glass ring, plastic ring
without and with thread) and culture chambers, which are especially useful for long-term cultures or
experiments. For more details or pricing information, please ask your local retailer.
6.1 MEA2100-CO2-C
The MEA2100-CO2-C is a climate chamber for MEA2100-Systems. Connect the chamber via magnetic
forces on the lid of a MEA2100 headstage to create a 5 % CO
2 atmosphere (humid or non humid)
around the biological probe. Connect a tube with an inner diameter of +/- 2.3 mm to the tube connector.
6.2 Sealed MEA Culture Dish
In order to allow long-term cultivation and recording, Multi Channel Systems recommends the use of teflon
membranes (fluorinated ethylene-propylene, 12.5 microns thick) developed by Potter and DeMarse (2001).
The ALA-MEA-MEM membrane is produced in license by ALA Scientific Instruments Inc., and distributed via
the worldwide network of MCS distributors.
The sealed MEA culture chamber with transparent semipermeable membrane is suitable for all MEAs with
glass ring. A hydrophobic semipermeable membrane from Dupont that is selectively permeable to gases
(O
2
, CO
2
), but not to fluid and H
2
O vapor, keeps your culture clean and sterile, preventing contaminations
by airborne pathogens. It also greatly reduces evaporation and thus prevents a dry-out of the culture.
Reference
 Reference: Potter, S. M. and DeMarse, T. B. (2001). "A new approach to neural cell culture for long-term
studies." J Neurosci Methods 110(1-2): 17-24.