Multichannel Systems MEA Manual - Manual (Page 12)

MEA Manual
12
4 MEA Types and Layouts
Various types of MEA biosensors are available for all kind of extracellular multi channel recordings.
Typical MEAs for in vitro applications have 60 microelectrodes arranged in an 8 x 8 layout grid embedded
in a transparent glass substrate. You can cultivate the tissue or cell culture directly on the MEA. EcoFlex-
and FlexMEAs are made for in vivo and in vitro applications.
MEA types differ in the materials used for the carrier and the recording area, and in the geometry, that is,
electrode size and interelectrode distances. The electrode size and interelectrode distances are used for
categorizing MEAs: The first number refers to the interelectrode distance, for example 200 μm, and the
second number refers to the electrode size, for example 10 μm, which results in the standard MEA type
200/10, for example.
Standard versions are available with an internal reference electrode (abbreviated “iR”) and with various
culture chamber interface options. Culture chambers are available with and without lid.
Please ask for custom layouts, that is, MEA layouts according to your specifications.
In this chapter, each MEA type is briefly described and noted.
 Standard MEAs with flat round TiN electrodes in an 8 x 8 layout grid for all applications.
 MEAs with 6 x 10 layout grid and 500 μm inter electrode distances.
 HighDenseMEAs with the highest spatial resolution and a double recording field of 5 x 6 electrodes each.
 HexaMEAs featuring a hexagonal layout, perfect for recording from retina.
 ThinMEAs with a "thickness" of only 180 μm, ideally suited for high-resolution imaging.
 Very cost efficient and robust EcoMEAs on glass or PCB (printed circuit board) base for applications with
lower spatial resolution and higher throughput, especially for established cardiomyocyte cultures, large
slices, or whole-heart preparations.
 Stimulation MEAs with 16 additional stimulation electrodes.
 Perforated MEAs allow perfusing the acute slice from up- and downside. For use with MEA2100
headstages equipped with perfusion element (MEA2100-PE) and MEA1060 amplifiers with perfusion
ground plate (MEA-PGP) or with.
 Small perforated MEAs with 32 recording and 12 stimulation electrodes specified for use with
MEA2100-32- and USB-MEA32-STIM4-System.
 6 well MEAs feature a round MEA layout, separated in six segments of 3 x 3 electrodes, like a pie-chart.
Using the 6 well MEA with macrolon triangle or round chamber ring, you have 6 separate culture chambers
on one MEA, for example, for drug application in a screening experiment.
 4 quadrant 1000 MEAs with electrode layout organized in four quadrants and a center line.
 256MEAs with 252 recording electrodes in a 16 x 16 layout grid for use with USB-MEA256-System.
 6 well MEAs for use with USB-MEA256-System. 252 electrodes in 6 blocks of 6 x 7 electrodes in a round
layout for use with 6 well macrolon triangle or round chamber rings. You have 6 separate culture chambers
on one MEA, for example, for drug application in a screening experiment.
 9 well MEAs for use with USB-MEA256-System. 256 electrodes in nine blocks of 26 recording, two
stimulation and reference electrodes each. Using the 9 well MEA with macrolon quadrant, you have
9 separate culture chambers, for example, for drug application in a screening experiment.
 120MEAs and a perforated 120pMEA with 12 x 12 layout grid for use with MEA2100-120-System only.