Multichannel Systems MEA Manual - Manual (Page 24)

MEA Manual
24
4.12 MEA with 6 Wells for Use with USB-MEA256-System
256-6wellMEA200/30iR-Ti
The 256-6wellMEA200/30iR-ITO has 256 electrodes and has to be used with the USB-MEA256-System.
Please refer to the USB-MEA256-System manual for detailed information.
The dimension of the glass carrier is 49 x 49 x 1 mm as usual. The MEAs with 6 wells are stable
in a temperature range from 0 ° - 125 °C.
The
256-6wellMEA contains 252 recording, 6 reference and four ground electrodes arranged in 6 electrode
blocks with a 6 x 7 layout grid for the recording electrodes. The reference electrode is around each block.
They are embedded in a transparent glass substrate. The contact to the amplifier is provided by a double
ring of contact pads around the rim of the MEA. The standard material for MEAs is also used for
256-6wellMEA : The electrodes are from titanium nitride (TiN) with a silicon nitride (SiN) isolator,
and contact pads and tracks are made of transparent indium tin oxide (ITO).
Using the
256-6wellMEA with macrolon ring, you have six separate culture chambers on one MEA,
for example, for drug application in a screening experiment.
The spacing between the recording electrodes in the 6 x 7 grid averages 200 μm between the electrodes.
The electrode diameter of 30 μm results in an impedance of approximately 30 - 50 k.
Using 256-6wellMEAs
The same material is used for standard MEAs and 256-6wellMEAs . Therefore, they are equally robust and
heat-stabilized. They can be autoclaved and coated with different procedures for cell and tissue cultures.