Multichannel Systems MEA Manual User Manual
Page 39
MEA Manual
39
PEI stock solution
0.05 – 0.1 % PEI dissolved in borate buffer.
Laminin solution
20 μg/ml laminin in plating medium.
Procedure
Note: It is necessary to thoroughly rinse off unbound PEI from the plates before use,
as dried PEI is toxic.
1.
Pipette 500 μl PEI solution onto the MEA. The recording field should be completely covered.
2.
Incubate at RT for 1 h, or at 4 °C over night.
3.
Remove the PEI solution and thoroughly rinse 4 x with distilled water.
4.
Air-dry the MEA.
5.
Sterilize with UV light for at least 1 h after coating.
6.
(Place a drop of sterile laminin solution onto the MEA and incubate for 30 min. Aspirate, do not rinse,
and directly seed your cells. Alternatively, mix the cells with laminin solution before plating.)
Literature
Ulrich Egert, Thomas Meyer (2004); Heart on a Chip — Extracellular multielectrode recordings from
cardiac myocytes in vitro, "Methods in Cardiovascular Research", S. Dhein and M. Delmar (eds.)
Lelong, IH, et al. (1992); J. Neurosci. Res. 32:562-568
5.4.3 Coating with Polyornithine (plus Laminin)
Poly-D-lysine can be used as an alternative for polyornithine.
Materials
Polyornithine
Laminin,
1mg/ml (Sigma-Aldrich,
Inc.,
L2020)
Polyornithine solution
500 μg/ml polyornithine in distilled water
Laminin solution
5 μg/ml laminin in plating medium or PBS (phosphate buffered saline).