Multichannel Systems MEA Manual User Manual
Page 44
MEA Manual
44
5.5.5 Cleaning of FlexMEAs
FlexMEAs made of polyimide foil have a temperature range from 10 – 125 °C. They can be sterilized
by autoclavation.
Please do not use an ultrasonic bath for FlexMEAs!
Rinse with distilled water first, optional with ethanol 70%.
5.5.6 Removing Nitrocellulose Coating
Note: It is very important that you clean MEAs that have been coated with nitrocellulose and remove
all biological material first before removing the coating. If you applied methanol on an uncleaned MEA,
you would rather fix the cell debris on the MEA than actually remove the coating.
1.
Directly after usage, biological material is rinsed off under running water and the MEA is cleaned with
pH-neutral cleaning agents or enzymatically if necessary.
2.
Rinse the MEA 2 x with methanol. If nitrocellulose is not sufficiently removed by rinsing, incubate the
MEA filled with methanol for 15 to 30 min to dissolve the cellulose nitrate.
3.
Rinse the MEA with distilled water.
5.5.7 MEA Cleaning with EDTA-Collagenase
Materials:
Collagenase Type I
(Sigma-Aldrich, Inc., C0130)
0.5 mM EDTA
Phosphate buffered saline (PBS)
(Gibco/Invitrogen,
14190-144)
Collagenase solution:
Dissolve collagenase type I in PBS at 20 U/ml.
Method:
1.
Fill the MEA culture chamber with 0.5 mM EDTA and incubate for 30 min.
2.
Rinse the chamber 3 times with PBS.
3.
Fill the MEA with collagenase solution and incubate for at least 30 min at 37 °C.
4.
Discard the collagenase solution and rinse the MEA with distilled water at least 3 times.
5.
Air dry the MEA, preferably under a laminar flow hood.