Multichannel Systems MEA Manual - Manual (Page 4)

MEA Manual
4
5 MEA Handling 35
5.1 Hydrophilic Surface Treatment 35
5.1.1 Plasma Cleaning 35
5.1.2 Protein Coating 35
5.1.3 Preculturing 36
5.2 Sterilization 36
5.2.1 Sterilization with Ethanol and UV Light 36
5.2.2 Steam Sterilization (Autoclavation) 36
5.2.3 Dry-Heat Sterilization 36
5.2.4 Sterilization with Hot Water 36
5.3 MEA Storage 37
5.4 MEA Coating 37
5.4.1 Coating with Nitrocellulose 37
5.4.2 Coating with Polyethyleneimine (PEI) plus Laminin 38
5.4.3 Coating with Polyornithine (plus Laminin) 39
5.4.4 Coating with Poly-D-Lysine (plus Laminin) 40
5.4.5 Coating with Poly-D-Lysine (plus Fibronectin) 41
5.4.6 Coating with Fibronectin 41
5.4.7 Coating with Collagen 42
5.5 Cleaning of used MEAs 43
5.5.1 General Recommendations for Cleaning MEAs 43
5.5.2 Cleaning of pMEAs 43
5.5.3 Cleaning of EcoMEAs 43
5.5.4 Cleaning of EcoFlexMEAs 43
5.5.5 Cleaning of FlexMEAs 44
5.5.6 Removing Nitrocellulose Coating 44
5.5.7 MEA Cleaning with EDTA-Collagenase 44
5.5.8 MEA Cleaning with Terg-A-Zyme 45
6 Culture Chamber Options 47
6.1 MEA2100-CO2-C 47
6.2 Sealed MEA Culture Dish 47
6.3 MEA Culture Chamber with Lid 48
6.4 Culture Chamber for 9-Well MEAs 48
6.5 Culture Chamber for 6-Well MEAs 48