Luminex xPONENT 3.1 Rev 2 - Manual (Page 45)

• % CV of microspheres - The measure of relative dispersion within the distribution.
%CV = 100 x Std Dev / Mean
• Standard Deviation - For calculating sample variability or dispersion, Luminex uses the
standard deviation formula.
• Peak - The value that is equal to the largest number of data points within the distribution.
For example, in data set {1,2,2,3,3,3,4,5}, 3 is the peak because it occurs the largest
number of times in the distribution list.
• Trimmed Count*
• Trimmed Mean*
• Trimmed%CV of microspheres*
• Trimmed Standard Deviation*
• Trimmed Peak*
• % CV of Replicates - The measure of relative dispersion within the distribution of results
for replicate samples.
%CV = 100 X Std Dev / Mean
• % Recovery - A measure of how accurately your observed results match your expected
results following regression analysis.
(Observed concentration) / (Expected concentration) x 100%
• Expected Result - The known or expected test result value for a standard or control.
• Control Range - Low - The lowest value for an assay control used to determine pass/fail
criteria for an assay.
• Control Range - High - The highest value for an assay control used to determine pass/fail
criteria for an assay.
• Normalized Net Median - For each analyte in a well the Normalized Net Median (NNM) =
(net median of analyte) / (net median of normalization bead)
• Units - The unit of measure for an analyte, for example, pg/mL.
*Trimmed statistics remove the lower and upper five percent of the extreme statistical values,
then use the remaining values for the Mean, Standard Deviation, or %CV calculations.
The statistics displayed change according to the analysis type selected.
• Analyte - Contains a list of analytes run in the batch. Select an analyte to view all statistics
for that analyte.
• Current Well - Displays the statistics of the well currently being displayed (This changes to
Displayed Well if viewing a batch using the Open button of the Saved Batches tab).
• Single Step - Allows analysis one well at a time. Click to turn function on or off.
• Results - Displays statistics related to the batch. Use the up, down, left, and right arrow
buttons to move through the table, or use the scroll bars. The maximize/minimize toggle
button expands the batch table and returns it to the standard size.
• Plate - A list of the available plates (if more than one).
WARNING: If using multiple plates, be careful to ensure that plates are used
in the proper order. Failure to do so can result in inaccurate data
and test results.
Histogram/Dot Plot - Displays real-time monitoring of batch sampling during acquisition.
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