Luminex xPONENT 3.1 Rev 2 - Manual (Page 19)

Gating information may change with a new lot of xMAP beads. Each time you receive a new
lot of xMAP beads, evaluate them with the current protocols. If gating information changes,
create a new protocol identical to the current protocol, but with a new version number and
new gating information. If running an xMAP-based kit, follow the instructions found on the
kit’s product insert or use the provided software protocol.
Plates
When using uncovered plates, use black opaque plates, if possible, to reduce
photobleaching.
For heated assays, use CoStar ® Thermowell ® 96-well, thin-wall polycarbonate, model P
plates.
For nonheated assays, select a 96-well plate with an overall height no greater than 0.75
inches (19 mm). See the
Bead Concentration section for additional information.
See the recommended consumables list on the Luminex website at http://
www.luminexcorp.com/Support/SupportResources/index.htm#tabs-4 for more information.
xPONENT ® 3.1 Rev. 2
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