Luminex xPONENT 3.1 Rev 2 - Manual (Page 37)

• Min MFI Enabled - Select this box to enable a minimum MFI for the Allele Call
analysis. Type a value in this box to set the minimum MFI for analysis.
• Number of Standards - Click to type the number of standards for the protocol.
• Number of Controls - Click to type the number of controls for the protocol.
• Fit of all Standards - The standard curve will be determined by using each individual
standard replicate when calculating the standard curve. For example if one runs
duplicates of a 7 point standard curve, the software will calculate the standard curve
by using 14 points.
• Mean of Replicates - The standard curve will be determined by averaging the
individual standard replicates when calculating the standard curve. For example if one
runs duplicates of a 7-point standard curve, the software will calculate the standard
curve by using 7 averaged points.
• Analyze Results While Acquiring Samples - The software allows for real-time
viewing of the results as the instrument analyzes samples.
• Use External Analysis Program - Select this check box to use a third-party program
to analyze the data. The Analysis Program list becomes active when this is selected.
• Analysis Program - Use this list to select which program to use for data analysis.
• Cancel - Click to return to the main Batches tab.
• Next - Click to advance to the Analytes tab.
Analytes Tab
Batches > Batches > Analytes
Use this tab to select or edit analytes used in the batch or protocol.
This tab contains the following:
Analyte grid - A grid representing each analyte from 1 to 50. Select All selects all analytes,
and Deselect All deselects all analytes. Click a numbered analyte to select it; click the
analyte again to deselect it. You can also click and drag to select groups of analytes.
Selected analytes are red. Deselected analytes are gray. An analyte marked as an intra-well
normalization bead is blue.
Default Analysis - The default analysis changes based on the Analysis Type selected in
the Settings tab. You can change the analysis settings for all analytes by clicking Change if
this button is enabled in this tab. This displays the Analysis Settings dialog box.
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