Luminex xPONENT 3.1 - Manual (Page 125)

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Using the Software
Edit Protocol
1. Open the Protocols page, then open the Protocols tab.
2. Select a protocol.
3. Click Edit. The Settings tab opens.
4. Define settings and click Next. The Analytes tab opens.
5. Define analytes and click Next. The Plate Layout tab opens.
6. Define the plate layout.
7. Click Save.
Lots and Kits
You can edit standard and control lot information. Once a lot is used, changing or
modifying it will prompt you for a new lot name.
For assay reagents specified in protocols, you can create new lots, edit lot information,
select pre-existing lots for reuse, import lots, and export lots.
Assay kits include standards and/or controls. Once you enter the assay kit information, it
can be used in multiple protocols. However, you should create separate kits specific for
use with each protocol.
Create Kit
1. Open the Protocols page, then open the Protocols tab. Click on the protocol that you
want to use for the kit, then click New Std/Ctrl. The Std/Ctrl Details tab opens.
2. Click on the protocol that you want to use for the kit, then click New Std/Ctrl. The Std/
Ctrl Details tab opens.
3. Type the name of the kit in the Name box, the lot number in the Std/Ctrl Kit Lot# box,
the expiration date using MM/DD/YY format in the Expiration box, and the
manufacturer in the Manufacturer box.
4. Click Apply Std Lot if you wish to apply a standard lot. The Select Lot dialog box
opens. Click a lot and select OK.
5. Click Apply Ctrl Lot to apply a control lot. The Select Lot dialog box opens. Click a lot
and click OK.
6. Alternatively, type the appropriate information in the Assay Standard Information and
Assay Control Information sections. The number of standards and/or controls in
these sections is defined in the protocol. If your batch uses controls, select Expected,
Low, or High from the Show Value options. Use the Apply Values arrows to apply
values down or across the range of analytes.
7. Click Save.
Create Lot
To create lots, you must use a protocol that uses either Quantitative or Qualitative
analysis settings.
1. Open the Protocols page, then open the Protocols tab. Click the Stds & Ctrls tab,
then click Create New Std/Ctrl Lots.
2. In the Select Protocol dialog box, select the protocol you want to use for this lot, then
click OK. The Std/Ctrl Details tab opens.