Appendix b. glossary – Bio-Rad Model 680 Microplate Reader Accessories User Manual
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Appendix B. Glossary
Assay: A set of wells grouped together for analysis.
Blank: A well used for background subtraction.
Endpoint analysis: Analysis based on a single absorbance value per well.
Kinetic analysis: Analysis of the rate of change of absorbance for a well over a set
period of time.
OD (Optical Density): A measure of absorbance.
Protocol: A group of settings that control a microplate reader. Includes incubator
controls, filter settings, mix times, etc. You adjust these settings in a dialog box, and
your adjustments may be saved as a file and used repeatedly.
Standard: A well whose concentration is known.
Sample/Unknown: A well whose concentration is to be determined.
Template: A graphical representation of a microplate used by Microplate Manager
to identify the numbers and types of wells, different assays, locations of standards,
etc. on the microplate
Well: An individual solution container on a microplate.
.MPM: The extension of a Data File name.
.EPR: The extension of a Endpoint Protocol File name.
.KPR: The extension of a Kinetic Protocol File name.
.MPR: The extension of a multiple Endpoint Protocol File name.