Bio-Rad Bio-Plex® 200 Systems User Manual
Page 38
Bio-Plex Manager has
Most likely:
detected a change in
sheath pressure.
Sheath reservoir cap not
Tighten sheath cap. Click OK.
on securely
Message should disappear within 2
min.
Sheath bottle lines are not
Make sure that all hoses are connected
connected properly
to the appropriate ports, and that they
clicked into place.
Less likely:
Sheath fluid level above
Adjust sheath fluid level so that sheath
the AIR port on the sheath
fluid is below the AIR port of sheath
container
bottle.
Sheath and waste bottle
Make sure that the blue-ringed cap is
caps are reversed
on the sheath bottle and the orange-
ringed cap is on the waste.
Sheath bottle has a leak
Try new sheath bottle. Call technical
service for further assistance.
HTF unit not turned on
Turn on HTF unit and ensure that
message disappears.
Air compressor not
Listen for air pump to turn on when
working
Warm Up is selected. Contact
Technical Support for further assistance.
Bio-Plex Manager has
Room temperature has
Calibrate array reader.
detected a change in the
changed
temperature of the array
reader. Please calibrate
before running on assay to
ensure accurate results.
The calibration was
Calibration procedure
Make sure Cal1 beads and Cal2
unsuccessful. Please repeat
failed
beads are placed in the appropriate
calibration. If calibration
wells (Cal1 in red well and Cal2 in
fails a second time, consult
green well). Repeat calibration. Make
Troubleshooting Guide.
sure you are using a clean MCV plate.
The calibration was
Calibration procedure
Run Unclog procedure, then repeat
unsuccessful. Bio-Plex
failed due to low bead
calibration. If problem persists, contact
Manager has detected a
number
Technical Support. See “low bead
problem with low bead
number” in troubleshooting guide.
number. Please repeat
calibration.
Optical Validation
Problem with optical
Recalibrate the array reader and then
Procedure shows value(s)
component of array
repeat validation procedure. If values
outside of acceptable
reader
are still out of range, contact Technical
range(s).
Support.
Reporter Validation
Problem with optical
Recalibrate the array reader and then
Procedure shows value(s)
component of reader
repeat validation procedure. If values
outside of acceptable
are still out of range, contact Technical
range(s).
Support.
Classify Validation
Problem with calibration
Recalibrate the array reader and then
Procedure shows value(s)
or optical component of
repeat validation procedure. If values
outside of acceptable
reader
are still out of range, contact Technical
range(s).
Support.
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Message/Problem
Causes
Solution