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Bio-Rad Bio-Plex® 200 Systems User Manual

Page 38

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Bio-Plex Manager has

Most likely:

detected a change in
sheath pressure.

Sheath reservoir cap not

Tighten sheath cap. Click OK.

on securely

Message should disappear within 2
min.

Sheath bottle lines are not

Make sure that all hoses are connected

connected properly

to the appropriate ports, and that they
clicked into place.

Less likely:

Sheath fluid level above

Adjust sheath fluid level so that sheath

the AIR port on the sheath

fluid is below the AIR port of sheath

container

bottle.

Sheath and waste bottle

Make sure that the blue-ringed cap is

caps are reversed

on the sheath bottle and the orange-
ringed cap is on the waste.

Sheath bottle has a leak

Try new sheath bottle. Call technical
service for further assistance.

HTF unit not turned on

Turn on HTF unit and ensure that
message disappears.

Air compressor not

Listen for air pump to turn on when

working

Warm Up is selected. Contact
Technical Support for further assistance.

Bio-Plex Manager has

Room temperature has

Calibrate array reader.

detected a change in the

changed

temperature of the array
reader. Please calibrate
before running on assay to
ensure accurate results.

The calibration was

Calibration procedure

Make sure Cal1 beads and Cal2

unsuccessful. Please repeat

failed

beads are placed in the appropriate

calibration. If calibration

wells (Cal1 in red well and Cal2 in

fails a second time, consult

green well). Repeat calibration. Make

Troubleshooting Guide.

sure you are using a clean MCV plate.

The calibration was

Calibration procedure

Run Unclog procedure, then repeat

unsuccessful. Bio-Plex

failed due to low bead

calibration. If problem persists, contact

Manager has detected a

number

Technical Support. See “low bead

problem with low bead

number” in troubleshooting guide.

number. Please repeat
calibration.

Optical Validation

Problem with optical

Recalibrate the array reader and then

Procedure shows value(s)

component of array

repeat validation procedure. If values

outside of acceptable

reader

are still out of range, contact Technical

range(s).

Support.

Reporter Validation

Problem with optical

Recalibrate the array reader and then

Procedure shows value(s)

component of reader

repeat validation procedure. If values

outside of acceptable

are still out of range, contact Technical

range(s).

Support.

Classify Validation

Problem with calibration

Recalibrate the array reader and then

Procedure shows value(s)

or optical component of

repeat validation procedure. If values

outside of acceptable

reader

are still out of range, contact Technical

range(s).

Support.

33

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Message/Problem

Causes

Solution