Bio-Rad Bio-Plex® 200 Systems - Manual (Page 28)

Note: You may use the additional waste line provided with the system to drain to a larger
waste container. The large waste container waste must be positioned so that it is no
more than 3 or 4 ft below the array reader. Please note that the instrument flow rate is
influenced by waste container placement, which may affect performance.
10. Lower the stainless-steel filter end of the sheath fluid line to the bottom of a full box of
sheath fluid. Secure the cap on the sheath fluid box. Position the sheath fluid container on
the floor so that the cap is on the top.
11. Turn on the power to the HTF; the HTF should automatically prime itself. You will hear the
HTF pump turn on. When the HTF reservoir is about 2/3 full, it will stop priming automatically.
12. Open the center access door on the array reader. Use a screwdriver to turn the regulator
screw fully clockwise (this may requre several full turns), then one half-turn back.
Fig. 21. Regulator screw adjustment.
13. From the Instrument menu select Additional Functions, and then Prime. During this
prime cycle use a screwdriver to adjust the regulator on the front of the HTF system (not
shown). Adjust it until the sheath pressure displayed in the Information Box reads the
same as the sheath pressure you recorded in step 6. This may take many turns. The
system should stabilize at this sheath pressure. The air pressure should be the same as
you recorded in step 6, within 0.1 psi.
If the Prime cycle ends before you have completed the adjustment, select Prime again and
continue to adjust the regulator.
14. When the pressure has been set it is critical that the flow rate be verified. Collect the
flowthrough to waste for 2 min in a graduated container capable of estimating volume to
0.5 ml. The volume collected should be 10.5 to 11.5 ml. Use universal precautions to avoid
contamination if the system has been used with human blood, body fluids or tissues. To
minimize the biological hazard, use the flow rate test kit available from Bio-Rad (catalog
#800-0502). If the flow rate is too low, increase the pressure by turning the regulator screw
on the HTF unit clockwise. If the rate is too high, decrease the pressure by turning the
regulator screw counterclockwise. Start by turning the regulator screw a quarter-turn in the
appropriate direction then retesting the flow rate. Continue until the flow rate is within the
specification.
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Regulator