Bio-Rad Bio-Plex® 200 Systems - Manual (Page 25)

2. Click the Eject icon. Insert the MCV plate into the microplate platform. Click Retract.
3. Choose Instrument from the main menu bar. Choose Additional Functions, followed by
Prime.
4. Inspect the waste line outside the instrument for air pockets. Repeat the priming procedure
until no air pockets are observed in the waste line outside of the array reader. This may
require a few priming cycles.
5. Choose Instrument from the main menu bar. Choose Additional Functions. Choose
Alcohol Flush. Wait for function to complete.
6. Choose Instrument from the main menu bar. Choose Additional Functions. Click
Wash. Wait for function to complete.
7. Repeat Wash two more times.
3.10 Resetting Instrument Pressure Settings
1. After the system has been primed, it is necessary to run the Reset Instrument Pressure
Settings utility for optimal instrument performance. The utility initially sets the Calibration
Pressure and the Manufacturing Pressure to the same value. Subsequently, whenever a
calibration is performed the Calibration Pressure will be updated, but it should not diverge
very far from the original Manufacturing Pressure setting unless there is a system problem.
The settings are displayed in the Instrument Info window under the Device Status tab.
2. The Reset Instrument Pressure Settings utility should be performed first using the
sheath fluid bottle attached to the system. If a HTF is included with the system, this utility
will be rerun in the HTF Setup section which follows.
3. With the array reader off and Bio-Plex Manager software closed, go the the Utilities folder
in Bio-Plex Manager 5.0 (go to Start > Programs > Bio-Plex Manager 5.0 > Utilities). Click
on Reset Instrument Pressure Settings. This will set up the system to set the instrument
pressures the next time Bio-Plex Manager is launched.
4. Power on the array reader and start Bio-Plex Manager software. As the program opens,
you will see a message that the system is Updating system settings. A message noting
that the settings were successfully updated will be displayed when the process is complete.
5. Restart the array reader and Bio-Plex Manager software.
3.11 HTF Setup
1. Unpack all components. Ensure that all accessories are supplied.
2. Start with the original sheath fluid and waste containers connected to the array reader. Check
that the sheath fluid container is filled just below the air intake valve.
3. Make sure the array reader is turned on, and start the Bio-Plex Manager software.
4. From the Instrument menu, select Additional Functions, then select Prime.
5. Open the Instrument Information window and select the Device Status tab (Figure 19).
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