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Bio-Rad Bio-Plex® 200 Systems User Manual

Page 37

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Pressurizing in status bar of

Leak in sheath bottle or

Tighten sheath cap or replace sheath

software.

cap

bottle.

Sheath and waste bottle

Make sure that the blue-ringed cap is

caps are reversed

on the sheath bottle and the orange-
ringed cap is on the waste bottle.

System pressure settings

Update pressure settings. Contact

incorrectly set

Technical Support for instructions.

Needle stuck in down

Protective assay plate

See hardware manual for procedure for

position.

covering was not removed

raising needle stuck in down position.
Then remove cover from assay plate.

Needle guide is not

Tighten needle guide by turning tube

screwed all the way in

clockwise until tight.

Sample needle is bent

Replace bent needle with a new
needle (Section 3.8.1, p. 16).

No assay signal detected.

Most likely:

Error in assay preparation

Make sure that streptavidin-PE was
added. See the assay kit manual.

Incorrect target values used

Check that target values in calibration

in calibration

dialog box match the values on Cal1
and Cal2 bottles.

Waste container overfilled

Empty waste. Reconnect waste.
Perform Unclog.

Sheath reservoir low or

Refill sheath reservoir. Perform

empty

Start Up.

Less likely:

Waste line not connected

Check waste line connection. Make

properly

sure that it clicks into place. Make sure
cap is vented and there is no pressure
inside the waste bottle.

Laser not functioning

Perform Calibrate using calibration kit.
Call Technical Support for further
assistance.

32

Message/Problem

Causes

Solution

www.bio-rad.com/bioplex/