Bio-Rad Bio-Plex® 200 Systems - Manual (Page 29)

15. Because the flow rate in step 14 is adjusted by altering the system pressure, it is necessary
to repeat steps 3 to 5 of Section 3.10 (Resetting Instrument Pressure Settings).
Note: It is necessary to run the Reset Instrument Pressure Settings utility whenever the flow
rate (or system pressure) is changed. This is a rare occurrence in normal operation.
3.12 Vacuum Manifold Setup
Bio-Rad recommends the use of the Millipore MultiScreen separations system (Millipore
catalog #MAVM0960R) for preparing Bio-Plex assays. A setup and validation procedure for this
apparatus is included here. More specific instructions for the setup of the apparatus as well as
specific product information may be found in the Millipore MultiScreen separations system user
guide. Note that depending on the type of samples used in Bio-Plex assays, the pressure
necessary to achieve optimal results may be different. If you choose to use your laboratory
(house) vacuum system, be aware that fluctuations in vacuum pressure may be extreme
enough so that you may need to purchase and integrate a pressure regulator. Alternatively, you
may also purchase a vacuum pump to ensure optimal steady-state vacuum pressure. Finally,
once you have calibrated your manifold, it is important to validate that this pressure is optimal
for performing Bio-Plex assays. Follow the verification procedure in this section closely for
optimal results using Bio-Plex assays.
Equipment: Required
• Vacuum source — laboratory vacuum or vacuum pump
• Pressure regulator (if extreme fluctuations in house vacuum are a problem)
• Millipore MultiScreen separations system (Millipore catalog #MAVM0960R)
• Flat-bottom microplate (not a filter plate)
• Millipore 96-well filtration plate (Millipore catalog #MSBVS1210)
• Phosphate buffered saline
• 8-channel pipet
3.12.1 System Setup
Setup of the MultiScreen Vacuum Manifold
Figure 22 illustrates the vacuum manifold setup and its attachment to a laboratory vacuum
source. For more specific details regarding the setup of the manifold, refer to the Millipore
MultiScreen separations system user guide.
Fig. 22. Vacuum manifold and vacuum source setup.
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