Bio-Rad Bio-Plex® 200 Systems - Manual (Page 24)

Fig. 17. Sample needle assembly.
12. By holding onto the needle height adjustment thumbscrew on the needle arm, manually
move the needle so that it just touches the bottom of the needle adjustment well of the
MCV plate. Move the needle up and down gently a couple of times to verify that the needle
is barely touching the bottom of the well.
13. Tighten the needle height adjustment thumbscrew so that it is no longer possible to manually
move the needle up and down. Take care to ensure that the needle does not move while you
are tightening the screw. Do not overtighten.
14. In the Adjust Needle window, click on the Up/Down button to move the needle up and
down. Look inside the microplate platform at the MCV plate. The needle should just touch
the MCV plate at the bottom of the cutout (use flashlight for better viewing). Readjust the
needle height if necessary.
15. When the needle is adjusted properly, click the Eject/Retract button.
16. Remove the MCV plate from the microplate platform and click Eject/Retract.
17. Click on the Save button in the Adjust Needle window.
18. Perform a Wash Between Plates step to remove any air introduced into the lines.
3.9 Initial System Priming
This procedure is to be performed only during the initial installation of the array reader.
1. Fill the maintenance, calibration, and validation (MCV) plate (Figure 18) with deionized
water and 70% isopropyl alcohol in the appropriate wells.
Fig. 18. MCV plate IV.
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Needle height adjustment
thumbscrew