Bio-Rad Bio-Plex® 200 Systems User Manual

Page 27

Fig. 20. HTF setup.

Make the following connections to connect the HTF to the array reader:

•

Connect the sheath fluid line (blue fitting) to the Sheath Out connector on the front of the
HTF. Lubricating the rubber O-rings with water facilitates attaching these connectors

•

Connect the air line (green fitting) to the Air In connector on the front of the HTF

•

Connect the sheath fluid line to the sheath fluid connector on the side of the array reader
(blue fitting)

•

Connect the air line to the air connector on the side of the array reader (green fitting)

•

If you are using the HTF waste line, connect the waste line tubing to the waste
connector on the side of the reader, and run the other end of the waste line into an
appropriate biohazard receptacle. Cut off excess tubing, and make sure the waste
receptacle is level with the array reader or no more than 3 ft below it.

Note:

The waste container must be vented.

•

Connect the sheath fluid intake line (white fitting) to the Sheath In connector in the
front of the HTF

•

Connect the communication cable to the DB9-PIN connector on the back of the HTF
system. Connect the other end to communication port 2 (P2) on the back of the
Bio-Plex array reader

•

Connect the power cord to the back of the HTF and plug the other end into an
approved outlet

Note: If you are using the 1.5 L waste bottle included with the Bio-Plex system, be sure to

empty it after every two plates are read.