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2 description of system components – Bio-Rad Bio-Plex® 200 Systems User Manual

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2.2 Description of System Components

The Bio-Plex 200 system is comprised of the following components:

Array reader — combines 2 lasers, fluidics, and real-time digital signal processing to distinguish
up to 100 different color-coded different color-coded bead sets, each representing a different
assay

Microplate platform — automates the reading of 96-well plates, yielding up to 9,600 data
points in ~ 35 min

PC and monitor — controls the Bio-Plex suspension array system via Bio-Plex Manager
software

MCV (maintenance, calibration, and validation) plate IV — automates the maintenance,
calibration, and validation functions of the array reader

Bio-Plex reservoir — allows maintenance functions to be seamlessly run before and after a
protocol resulting in improved walkaway capability

Calibration kit — contains beads to standardize daily signal output and ensure unit-to-unit
reproducibility of the reader

Validation kit — contains beads to validate the operational specifications of the reader,
including accuracy, linearity, dynamic range, slope, fluidics, and optical alignment

Optional HTF — delivers up to 20 L of sheath fluid without user intervention

Sheath fluid cube — contains 20 L of sheath fluid (1x) for the array reader

2.2.1 Array Reader

The array reader (Figure 1) is a compact flow analysis unit integrating a dual laser detection
system, optics, fluidics, and advanced digital signal processing. When used with the microplate
platform, the array reader facilitates the simultaneous analysis of up to 100 different analytes
from a single sample. The features of the array reader are outlined in Table 1.

Fig. 1. Array reader – front and side panel features.

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Fluid connection
(side of reader)

Cheminert fitting

Sample arm

Sample needle

Needle height adjustment
thumbscrew

Access doors