Bio-Rad CHEF Mapper® XA System User Manual

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6.2 Hardware and Software Requirements

The interactive program disc operates with Microsoft Windows 3.1 on IBM or compatible

computers of the AT class (80386) or faster. Generally, if a computer will operate with
Windows software, this program will run, but you should contact Bio-Rad before making a
specific computer purchase. The minimum RAM required is 512 K. A color monitor and
EGA or VGA graphics card are recommended. Monochrome systems operating with
Windows will generally give satisfactory displays.

6.3 Operation

This section assumes you are familiar with Microsoft Windows software. Consult the

Microsoft Windows manual for the basic techniques of using the mouse and dialog boxes.

Set-up

1. Insert the Algorithm start-up disc.

2. Open the Algorithm by double-clicking on the application or selecting it and choosing the

Open command from the file menu. The window displays the Menu Bar and control
points for programming, printing, and transmitting.

3. Choose Set-up from the File menu. The Default Program Mode window will appear.

Select Fully Automated or Semi-Automated Mode. The algorithm will default to whichever

mode is selected. It is not necessary to open this window except to change the default mode.

Algorithm

1. Choose Algorithm from the file menu. The CHEF Mapper Interactive Algorithm

dialog box will appear.

2. Select the Mode command. Toggle between Fully Automated and Semi Automated

Mode by clicking the Mode button. Fully Automated Mode allows entries of the
Minimum DNA Size and m Maximum DNA Size only, with other options inactive. The
Semi-Automated Mode allows choices of all options.

3. Select Semi-Automated Mode or Fully Automated Mode.

4. Enter the smallest size of DNA in kb in Minimum DNA Size either directly into the edit

field or with the slide bar.

5. Enter the largest size of DNA in kb in Maximum DNA Size either directly into the edit

field or with the slide bar. For Fully Automated Mode, continue with step 10.

6. Select the type of agarose by clicking the button next to the agarose type in Agarose Type.