Bio-Rad CHEF Mapper® XA System - Manual (Page 18)

Store Program (STORE PGM) stores approximately 20 user programs
(see Section 4.8).
Stop Run, activated by pressing START RUN and PAUSE/CONT
simultaneously, stops the run and displays the message “You will halt
the program and clear it”. Enter 0 (for No) to put the run into the pause
mode. Enter 1 (for Yes) to clear the program. (Note: The high voltage
light will take 5–25 seconds to go off, to allow the power supply time to
power down.) Enter 2 to go to the editing mode (see Section 4.9).
Start Run is used to begin the electrophoresis run. When START RUN
is pressed, the high voltage, AC power, and run on indicator lights will
come on. IMPORTANT: Never turn the CHEF Mapper power switch
off when the high voltage light is on. Use STOP RUN first to clear the
program, then turn the power off after the high voltage light goes off.
Pause/Cont may be pressed during a run to pause the run. The run pause
indicator light comes on and the run on light goes off. The high voltage
light takes 5–25 seconds to go off, to allow the power supply time to
power down. To start the run, press PAUSE/CONT again.
Comma is used to escape any program. Pressing COMMA for 6 sec-
onds (six beeps) will display the message “Total Initialization - All
memory will be erased - Go on?” Enter 0 (for No) to put the run into
the pause mode. Enter 1 (for Yes) to clear the program. Entering 1 will
erase every stored user program. Be sure you really want to erase every-
thing before answering yes. (Note: The high voltage light will take 5–25
seconds to go off to allow the power supply time to power down.) Enter
2 (for Edit) to enter the editing mode (see Section 4.9).
4.3 FIGE Mode
Entering Run Parameters into FIGE
In field inversion gel electrophoresis (FIGE), the two electric fields are 180° apart. FIGE
is useful in resolving small DNA fragments, less than 100 kb. The FIGE key sets the CHEF
Mapper system to perform field inversion electrophoresis. The FIGE mode can be pro-
grammed in a variety of ways, including conventional FIGE, or with forward and reverse
fields of different voltages and different switch times. It is also possible to program linear or
nonlinear switch time ramps in the FIGE mode (see Section 9.2 for ramping).
To run gels in the FIGE mode, enter voltage gradient, switch time (ramp or no ramp),
and run time at the appropriate prompts. The prompt moves sequentially from one parame-
ter to the next after ENTER is pressed. The parameters must be entered separately for the
forward and reverse directions. When FIGE is pushed, the following screen is displayed:
Forward Voltage Gradient = [
] V/cm
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STORE PGM
START RUN PAUSE/CONT
STOP RUN
START RUN
PAUSE/CONT
,