Bio-Rad CHEF Mapper® XA System - Manual (Page 20)

F. Ramp: a =: The forward ramp display will appear only if there is a difference between ini-
tial and final switch times. The value “a” is the ramping factor and determines the
mathematical shape of the ramp. For more information on the ramping factor, see Section
9.2. To run a linear ramp, press ENTER at the a= prompt.
R. Ramp: a =: The reverse ramp display will appear only if there is a difference between the
initial and final switch times. Enter a value for “a”, and press ENTER.
After the values are entered for this screen, the CHEF Mapper system will display the mes-
sage: A Program is in memory. Please enter another command. The program that was
just entered is now in short term memory (RAM). If the CHEF Mapper system is turned off,
the program will be lost. The following options are available:
Start Run: Press START RUN to begin the run. If power goes off during a run, the pro-
gram is saved and resumes when power is restored. When the run is completed, only the
AC power light will be on, and the screen displays the message Run is Completed. Press
1 to save, or 0 to clear it. If you do not want the program saved, enter 0. If you want the
program saved, enter 1. The program can be rerun until power is turned off. To store the
program, press STORE PGM (see Section 4.8).
Edit the Program: To check the program just entered for errors or to make corrections,
see Section 4.9.
Store Program: To store the program just entered so that it is available for future use, see
Section 4.8.
Delay Start: To enter a time delay before the run is started, see Section 4.7.
Remove Program and Set Up a New Program: To remove the program just entered,
press either AUTO ALGORITHM, FIGE, TWO STATE, or MULTI STATE. The mes-
sage You will destroy last Program - Go On? will appear. Choose 0 for No, 1 for Yes,
or 2 for edit the program. Press 1 then ENTER to delete the program. Entering 0 saves the
program.
Running FIGE
While a FIGE program is being run, the following screens are displayed:
Screen 1:
Run time: The total run time, in hours and minutes.
Remaining: The time remaining to the end of the run (i.e., a countdown clock).
ma: The current in milliamperes.
Sw. time: The present switch time. This will change during a ramp.
Forward or reverse: Present field direction.
V/cm: The value of the voltage gradient.
Run Time = hh:mm
Remaining = hh:mm ma
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