Bio-Rad CHEF Mapper® XA System - Manual (Page 19)

Forward Voltage Gradient: Enter the forward voltage gradient with the number key pad.
Press ENTER. The total voltage applied across the electrodes is obtained by multiplying the
gradient by 33.5 (33.5 cm is the distance across the hexagonal electrode array). The allowable
voltage gradient range is 0.6–9.0 V/cm in 0.1 V/cm increments.
Int. Sw. Tm: To enter the initial switch time for the forward direction, enter the numeric
value, press HOURS, MINUTES, or SECONDS, then ENTER. The acceptable switch time
range is 0.05 seconds–18 hours. Enter the switch time as one time unit, e. g., enter 3 minutes
and 30 seconds as 3.5 minutes or 210 seconds.
Note: If a mistake is made while keying in a value, press CLR ENTRY and start again.
If the value has been entered before the mistake is found, use the editing mode to make
a correction (see Section 4.9).
Fin. Sw. Tm: To enter the final switch time for the forward direction, enter the numeric value,
press HOURS, MINUTES, or SECONDS, then ENTER. The acceptable switch time range is
0.05 seconds–18 hours. Enter the values in the same way as the forward initial switch time. If
the final switch time is the same as the initial switch time, press ENTER without re-entering
the time.
After the data for the forward gradient are entered, the following screen will appear for
the reverse gradient:
Reverse Voltage Gradient: Enter the reverse voltage gradient value, and press ENTER. The
reverse voltage gradient has an allowable range of 0.6–9.0 V/cm in 0.1 V/cm increments.
Int. Sw. Tm: To enter the initial switch time for the reverse direction, enter the numeric value,
press HOURS, MINUTES, or SECONDS, and ENTER. The acceptable switch time range is
0.05 seconds–18 hours.
Fin. Sw. Tm: To enter the final switch time for the reverse direction, enter the numeric value,
press HOURS, MINUTES, or SECONDS, and ENTER. The acceptable switch time range is
0.05 seconds–18 hours. If the final switch time is the same as the initial switch time, press
ENTER without reentering the time.
After the data for the reverse gradient are entered, the following screen will appear:
Total Run Time: Enter the total run time for the FIGE run, and press ENTER. The allowable
run time range is 1 minute–999 hours. Enter the run time as one time unit, either hours or
minutes. When entering the run time, first put in the numeric value, press HOURS or MIN-
UTES, then ENTER.
Total Run Time = [ . ]
F.Ramp: a = [ . . ]
Reverse Voltage Gradient = [ ]V/cm
Int.Sw.Tm = [ . . ]
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