Bio-Rad CHEF Mapper® XA System - Manual (Page 10)

Fig. 2.1. Interconnections between components of the CHEF Mapper system.
After making sure the power module is off, attach the power cords for the power module
and Cooling Module to the back of each instrument. Connect the 25-pin cable from the
electrophoresis chamber to the port labeled OUTPUT TO ELECTROPHORESIS CELL on
the front panel of the power module. Connect the coiled interlock cable from the
electrophoresis chamber to the jacks labeled TO INTERLOCK on the power module.
To connect the cell to the Cooling Module, attach approximately 1–2 feet of 1¼4 inch
internal diameter Tygon tubing to both the Flow In and Flow Out ports on the Cooling Module,
and secure the tubing with plastic clamps (provided). Connect a quick release connector (pro-
vided) to 2 feet of 3¼8 inch internal diameter Tygon tubing. Attach the quick release connector
to the left front port of the cell. Attach the other end of the 3¼8 inch tubing to the 1¼4 inch
tubing from the Flow In of the Cooling Module using a 3¼8 to 1¼4 inch reducer (provided).
The pump should be positioned between the outlet of the Cooling Module and the inlet (rear)
of the electrophoresis cell. Connect the 1¼4 inch tubing from the Flow Out of the Cooling
Module to the inlet of the pump using a 3¼8 to 1¼4 inch reducer. Connect approximately
two feet of 3¼8 inch Tygon tubing to the outlet of the pump using a 3¼8 to 3¼8 straight con-
nector (provided). Connect a quick release connector to the other end of the 3¼8 inch tubing.
Connect the quick release connector to the inlet of the cell.
Connect a quick release connector to a six inch piece of 3¼8 inch Tygon tubing, and
connect it to the right front port of the cell. This tube will be used to drain the buffer in the
electrophoresis cell.
Connect the 9 pin gray temperature probe cable (included) from the back of the
electrophoresis cell to the Remote Sensor port on the back of the Cooling Module.
Establish the correct buffer flow before attempting any electrophoresis runs. After estab-
lishing the flow rate, use that setting on the pump for electrophoresis runs. Fill the chamber
with 2.2 liters of buffer. The optimal buffer flow rate through the electrophoresis chamber is
approximately 1 liter per minute (70–100 setting). Turn on the pump, and measure the flow
of buffer at the right front port. Adjust the buffer flow with the pump.
Fine-tune the Cooling Module before attempting electrophoresis runs. Turn on the cooler
and pump approximately 0.5 hour before adjusting the temperature. Initially, it will be
necessary to fine-tune the temperature setting to achieve a consistent running temperature.
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