Bio-Rad CHEF Mapper® XA System - Manual (Page 25)

Note: If no interrupts are chosen, interrupts cannot be used for any of the blocks 1–8. If
you don’t want interrupts for Block 1, but want interrupts for some later block, enter 1
(yes) for interrupts.
The first example shows how to enter run parameters in the multi state mode with no
interrupts. Next, an example for entering run parameters in multi state with interrupts is shown.
Blk1 Runtim: Enter the total run time for Block 1, and press ENTER. The acceptable run time
range is 1 minute–999 hours. Enter the run time as one time unit, either hours or minutes.
When entering the run time, put in the numeric value, then press HOURS or MINUTES.
Note: If a mistake is made while keying in a value, press CLR ENTRY, and start again.
If the value has been entered and then the mistake is found, make the correction using
the editing mode (see Section 4.9).
After the data for the first screen are entered, the following screen will appear:
V/cm: Enter the voltage gradient for Block 1, State 1 using the number key pad, and press
ENTER. The total voltage applied across the electrodes is obtained by multiplying the gradient
by 33.5 (33.5 cm is the distance across the hexagonal electrode array). The allowable voltage
gradient range is 0.6–9.0 V/cm in 0.1 V/cm increments.
Ang: Enter the angle, then press ENTER. The angle entered must be in degrees and in the
range from 0 to + or - 180°.
In Tm: Enter the numeric value for initial switch time for State 1, press HOURS, MINUTES,
or SECONDS, then ENTER. The allowable switch time range is 0.05 seconds–18 hours.
Enter the switch time as one time unit, e. g., enter 3 minutes and 30 seconds as 3.5 minutes
or 210 seconds.
Fn Tm: Enter the numeric value for the final switch time for State 1, press HOURS, MINUTES,
or SECONDS, then ENTER. The allowable switch time range is 0.05 seconds–18 hours. Enter
the values in the same way as the initial switch time. If the final switch time is the same as the
initial switch time, press ENTER.
a =: The value “a” is the ramping factor and determines the mathematical shape of the ramp.
The ramping factor is active only if there is a difference between initial and final switch times.
For information on the ramping factor, see Section 9.2. To run a linear ramp, press ENTER.
Blk1 St01 [ . ]V/cm a = [ ]
Ang = [ . ]
Blk1 Runtim - [ ] Intrp = [ ]
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