Bio-Rad CHEF Mapper® XA System - Manual (Page 14)

15. At Continue with another state?, enter 0 for no. Then Continue with another block?,
enter 0 for no. The display is now A program is in memory.
3.5 Storing and Recalling a Program in Memory
In this exercise, you will store the previous program in memory. This operation must be
performed before pressing START RUN or beginning any other operations.
1. Press STORE PROGRAM on the panel.
2. The message Enter stored User Program number: is displayed. Press 1, then ENTER
to continue. The program will be stored under program number 1.
3. To recall the program into working memory, press USER PROGRAM on the panel. The
display shows You will destroy last program - go on? Enter 1 for yes. This removes the
last program in working memory. It does not erase any stored programs.
4. After Enter stored program number or Enter to abort, enter the number assigned in step 2.
5. Begin electrophoresis with START RUN. To view the program, press BLOCK before
pressing START RUN. It will respond with Enter Block Number:. Press 1. The first
line of the program will appear. You may go through the program and edit it. Press
ENTER or the cursor keys as many times as needed to get to the end of the program.
Section 4
The CHEF Mapper User Interface Display
4.1 Definitions
Terminology is derived from Clark et. al .32
State or Field State: Electric field vector, defined by voltage, angle, and duration. The
direction of the vector defines the path taken by DNA within the gel at any moment in response
to the field.
Block: A sequence of repeatable vectors (field states) which is repeated for a specified time.
From 1 to 15 states comprise a block.
Program: A sequence of 1–8 blocks making up a run.
FIGE Mode: Field Inversion gel electrophoresis, in which the two electric field vectors are 180° apart.
Two State Mode: Operating mode consisting of two field vectors, with each vector having
the same voltage and duration, but separated in direction by a definable included angle.
Multi State Mode: Operating mode consisting of up to 8 blocks, each consisting of 1–15
field vectors of definable angle, voltage, and duration.
Included Angle: The angle between the two vectors in the two state mode; measured relative
to the top of the gel.
Multi State Angle: The angle between a vector and the vertical, relative to the bottom of the gel.
Linear Switch Time Ramp: A linear increase or decrease in the duration of a field state
from the beginning to the end of a block. The steepness or slope of this ramp is dependent on
the difference between the initial and final switching times and total run time.
Nonlinear Switch Time Ramp: A switch time ramp that changes in a nonlinear fashion.
Voltage Gradient: Voltage across electrodes on opposite sides of the hexagon, a distance of
33.5 cm.
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