Opt 12 well/ exp,sqr, Uniform 96 well/ exp, sqr, Uniform 24 well/ exp,sqr – Bio-Rad Gene Pulser MXcell™ Electroporation System User Manual
Page 48
Mixed
Protocols
39
Opt 12 well/ Exp,Sqr
Table 14 shows the parameters that vary in each well for a 12-well plate. The protocol varies
in a wide range of starting condition for exponential and square waveforms in well sets.
Uniform 96 well/ Exp, Sqr
Table 15 shows the parameters that vary in each well for a 96-well plate. The protocol varies
the starting conditions for exponential and square waveforms in well sets:
•
Well sets ABCD 1-6 and EFGH 1-6: Exponential, 250 V, 350 μF, 1000
Ω
•
Well sets ABCD 7-12 and EFGH 7-12: Square wave, 250 V, 20 ms, 1000 μF, 1000
Ω
Uniform 24 well/ Exp,Sqr
These are parameters that vary in each well for a 24-well plate. Except for the larger well
size, the protocol is the same as “Uniform 96 well/ Exp, Sqr” on page 39.
Table 14. Opt 12 well/ Exp, Sqr conditions that vary across the plate.
Exponential wave
Square wave
1
2
3
4
5
6
7
8
9
10
11
12
A
200 V
350 μF
300 V
350 μF
400 V
350 μF
250 V
200 μF
250 V
350 μF
250 V
500 μF
200 V
20
ms
250 V
20
ms
300 V
20
ms
250 V
15
ms
250 V
20
ms
250 V
25
ms
B
C
D
E
F
G
H
Table 15. Uniform 96 well/ Exp conditions that vary across the plate.
Exponential wave
Square wave
1
2
3
4
5
6
7
8
9
10
11
12
A
250 V
350
μF
250 V
20ms
B
C
D
E
F
G
H