Bio-Rad Gene Pulser MXcell™ Electroporation System - Manual (Page 45)

Gene Pulser MXcell™ System Manual | Pre-Set Protocols
36
Opt 96 well/ Sqr, NP, D
Table 10 shows the parameters that vary in each well of a 96-well plate. The protocol
includes well sets 1–4 with these parameters: Square Wave, 250 V, 2,000 μF, 1000
Ω, 7–20
ms, and 1–3 pulses (NP).
Whole Plate Protocols
Whole plate protocols program all the wells in an electroporation plate with the same
conditions. The following is a list of the protocol names and conditions for each of the
whole plate protocols:
• 96 wells/ xp: Exponential wave, 250 V, 350 μF, and 1,000
Ω
• 24 wells/ Exp: Exponential wave, 250 V, 350 μF, and 1,000
Ω
• 12 well/ Exp: Exponential wave, 250 V, 350 μF, and 1,000
Ω
• 96 well/ Sqr: Square wave, 250 V, 2,000 μF, 1,000
Ω, and 20 ms
• 24 well / Sqr: Square wave, 250 V, 2,000 μF, 1,000
Ω, and 20 ms
• 12 well/ Sqr: Square wave, 250 V, 2,000 μF, 1,000
Ω, and 20 ms
Table 10. Opt 96well/ Sqr, NP, D conditions that vary across the plate.
1 2 3 4 5 6 7 8 9 10 11 12
A 20 ms
1 NP
15 ms
2 NP 10 ms
2 NP 7 ms
3 NP
B
C
D
E
F
G
H