Bio-Rad Gene Pulser MXcell™ Electroporation System - Manual (Page 29)

Gene Pulser MXcell™ System Manual | Program and Run the System
20
Turning on the System
To turn on the Gene Pulser MXcell system, press the power button on the right side of the
power module (Figure 2 on page 6).
Once initiated, the Gene Pulser MXcell system runs a series of tests. These test verify that
the system is running within specifications. The tests check the PulseTrac™ system
(page 49) and the firmware. During the tests the system displays the Bio-Rad Laboratories
logo, the Gene Pulser MXcell system name, and the firmware version.
After initialization, the home screen displays (page 10). From this screen it is easy to access
all options, including the first four options for programming and running protocols. To select
an option in the home screen, press the up and down arrow keys to select an option, and
press
ENTER to confirm the selection.
TIP: To return to the home screen from anywhere in the program, press the HOME
key on the control panel.
Protocol Set-Up Option
The Protocol Set-up option allows you to program protocols for the plate as follows:
• Whole plate (WHOLE PLATE): Apply the same pulse parameters to the entire
electroporation plate (page 20).
• Well set (WELL SET): Apply parameters to one well set within the electroporation
plate (page 21).
W HOLE P LATE P ROGRAMMING
To program a whole plate, follow these instructions:
1. Select Protocol Set-up in the home screen.
Then press ENTER to confirm the selection.
2. Select a plate size.
Use the arrow keys to select the Plate (96, 24 or 12), and press ENTER to confirm the
selection.
3. Select WHOLE PLATE.
Press ENTER to confirm the selection:
4. Select a waveform.
Press the arrow keys to select the waveform ( EXP or SQR), and press ENTER to confirm
the selection.
5. Enter the parameters.
Enter the required parameter values for the pulse units.
NOTE: If the values you enter for a parameter is outside the limits of the Gene
Pulser MXcell system, the value will change to the closest permitted value.
Plate:
96
24 12
Program:
WHOLE PLATE
WELL SET