Bio-Rad Gene Pulser MXcell™ Electroporation System - Manual (Page 22)

Well Sets and Quadrants in Electroporation Plates
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When programming a protocol, the well set appears with the letters of the rows, followed by
the column number. For example the well set “ABCD1” includes wells A, B, C, and D in
column 1 (Figure 8).
WARNING! All wells in a well set must be filled with either sample or sample
buffer. For example, if you want to electroporate six wells, fill a complete well set
(such as ABCD1) with sample and fill two wells in a second well set (such as
AB2) with sample. Finally, be sure to fill the remaining two wells in the second
well set (such as CD2) with the sample buffer.
Quadrants
The 24- and 96-well plates can be programmed in quadrants. A quadrant in a 96-well
electroporation plate includes a group of six well sets. In a 24-well plate each quadrant
consists of six groups of wells. Programming quadrants is an easy way to replicate gradient
experiments. Figure 9 shows the quadrants in both the 96-well and 24-well electroporation
plates.
Figure 9. Quadrants in 96- and 24-well plates.
96-well plate 24-well plate
One well set One well