Bio-Rad Gene Pulser MXcell™ Electroporation System - Manual (Page 42)

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Mixed protocols (page 38)
13. Opt 96 well/ Exp,Sqr 96 Use with cell lines with no protocol reference.
This protocol includes a range of average
starting conditions.
14. Opt 24 well/ Exp,Sqr 24 Use with cell lines with no protocol reference.
This protocol includes a range of average
starting conditions.
15. Opt 12 well/ Exp,Sqr 12 Use with cell lines with no protocol reference.
This protocol includes a range of average
starting conditions.
16. Uniform 96 well/ Exp,Sqr 96 Use with a set of defined conditions to
compare different cell lines and
electroporation of different molecules within
the same or different cell lines.
17. Uniform 24 well/ Exp,Sqr 24 Use with a set of defined conditions to
compare different cell lines and
electroporation of different molecules within
the same or different cell lines.
18. Uniform 12 well/ Exp,Sqr 12 Use with a set of defined conditions to
compare different cell lines and
electroporation of different molecules within
the same or different cell lines.
19. Mixed 96 96 Use for mixing different waveforms.
Alternating rows of exponential (250 V/350 uf)
and square waves (250 V/20 ms).
20. Mixed 24 24 Use for mixing different waveforms.
Alternating rows of exponential (250 V/350 uf)
and square waves (250 V/20 ms).
21. Mixed 12 12 Use for mixing different waveforms.
Alternating rows of exponential (250 V/350 uf)
and square waves (250 V/20 ms).
Table 6. List of pre-set protocols and their uses.
Protocol name
Number
of wells
When to use this protocol